Journal: The World Allergy Organization Journal

Article Title: A prospective cohort study of ultra-rush subcutaneous immunotherapy in dust mite-induced allergic rhinitis ☆

doi: 10.1016/j.waojou.2026.101334

Figure Lengend Snippet: Levels of cytokines after treatment. Blood EOS counts (A) and percentages (B) measured at months 0, 6, and 12 (M0, M6, M12). (C–F) Serum concentrations of the type 2 cytokines IL-4 (C), IL-5 (D), IL-13 (E), and the alarmin cytokine IL-33 (F) across the study timepoints. (G) Serum concentrations of IL-10. Data are presented as mean with 95% CI. Statistical comparisons between the Ultra-Rush and Cluster groups at each time point are indicated as ∗∗∗ P < 0.001, ∗∗ P < 0.01, and ∗ P < 0.05. EOS, eosinophils; IL, interleukin; CI, confidence interval

Article Snippet: The levels of interleukins (IL-4, IL-5, IL-10, IL-13, IL-33) were determined using standardized assay kits (Boster Biological Technology, Wuhan, China).

Techniques:

Journal: The World Allergy Organization Journal

Article Title: A prospective cohort study of ultra-rush subcutaneous immunotherapy in dust mite-induced allergic rhinitis ☆

doi: 10.1016/j.waojou.2026.101334

Figure Lengend Snippet: Correlation analyses of changes in immunologic parameters and clinical symptom scores. (A) Correlation between the change from baseline in EOS count and the change in VAS score at month 12. (B) Correlation between the change from baseline in EOS percentage and the change in VAS score at month 12. (C) Correlation between the change from baseline in sIgG4 levels and the change from baseline in IL-10 levels at month 12. Data represent the change between baseline (M0) and the 12-month endpoint (M12). Statistical analysis was performed using Pearson correlation, with the correlation coefficient (R) and P-value indicated on each panel. EOS, eosinophils; VAS, Visual Analog Scale; IL, interleukin; sIgG4, specific immunoglobulin G4

Article Snippet: The levels of interleukins (IL-4, IL-5, IL-10, IL-13, IL-33) were determined using standardized assay kits (Boster Biological Technology, Wuhan, China).

Techniques:

Journal: Frontiers in Veterinary Science

Article Title: Evaluation of the immunogenicity of a Crimean-Congo hemorrhagic fever virus vaccine candidate in mice developed based on a baculovirus Zera nanoparticle delivery system

doi: 10.3389/fvets.2023.1126785

Figure Lengend Snippet: Analysis of immunogenicity in mice. Analysis of induced IgG responses in mice by indirect ELISA for the Gn recombinant protein (A) and the Np recombinant protein (B) . The y-axis represents the OD450 nm of serum samples collected at 0, 14, 28, 35 and 42 d after immunization (DAI) for each group, *** p < 0.001, with significant differences from PBS and rvAc-dual (Bonferroni test). (C) Results of lymphocyte proliferation experiments. The y-axis represents the stimulation index of splenic lymphocyte samples collected at 35 and 42 DAI. ns: no significant difference; *** p < 0.001, significant difference (Bonferroni test). Quantitative analysis of IL-4 (D) and TNF-α (E) levels in the serum of immunized mice. All analyses were performed in triplicate, and error bars show the standard deviation (SD).

Article Snippet: TNF-α and IL-4 cytokines were detected in mouse sera by a commercial ELISA kit (Mouse IFN-γ/IL-4 ELISA BASIC kit Boster), and all sera to be tested were made in three replicates.

Techniques: Immunopeptidomics, Indirect ELISA, Recombinant, Standard Deviation

Journal: Journal of Nanobiotechnology

Article Title: Anti-inflammatory and immunomodulatory effects of the extracellular vesicles derived from human umbilical cord mesenchymal stem cells on osteoarthritis via M2 macrophages

doi: 10.1186/s12951-021-01236-1

Figure Lengend Snippet: Protective effect of M2 macrophages induced by hUCMSCs-EVs on OA chondrocytes in vitro. A IL-1β-induced OA chondrocytes were co-cultured with the supernatant of M2 macrophages (M2S) induced by hUCMSCs-EVs, or platelet-rich plasma (PRP) for 48 h, relative mRNA expression of the key genes TNF-α, MMP13, SOX9, and ACAN was measured by quantitative RT-PCR analysis; the experiment was performed triplicate; *p < 0.05, **p < 0.01, ***p < 0.001. B Western blot was performed to evaluate the expression of TNF-α, MMP13, and IL-4 proteins in PBS, M2S, or PRP-treated OA chondrocytes; GAPDH was employed as the loading control; *p < 0.05, **p < 0.01, ***p < 0.001. C The influence of M2S or PRP on the viability of chondrocytes was detected by the cell live/death experiment; green represents live cells while red represents dead cells; Scale bar: 1 mm

Article Snippet: And then these polyvinylidene fluoride membranes were incubated with first antibodies, anti-IL-4 (Boster, 10K274), anti-TNF-α (Proteintech, 17590-1-AP), anti-MMP13 (Proteintech, 18165-1-AP), anti-CD63 (Bioss, bs-1523R), anti-TSG101 (Abclonal, A1692), anti-CD81 (Bioss, bs-6934R), anti-CALNXIN (Proteintech, 10427-2-AP), or anti-GAPDH (Sangon Biotech Co., Ltd, China) at 4 °C for 12 h after blocking with 5% milk.

Techniques: In Vitro, Cell Culture, Expressing, Quantitative RT-PCR, Western Blot